Biphasic culture system for rapid Campylobacter cultivation

Conventional Biphasic Broth Blood Culture System

In a comparative fungal blood culture study, a lysis-centrifugation system (Isolator; Du Pont Co., Wilmington, Del.) detected 89% of all episodes of fungemia; the lysis-centrifugation system detected fungemia exclusively or significantly earlier than did a biphasic brain heart infusion bottle system 83% of the time. The lysis-centriguation system was particularly useful in the early detection o...

Biphasic system for growing bacteria in concentrated culture.

The concentration of masses of pathogenic bacteria can be simplified by growing them in a confined environment with diffusional access to a reservoir of nutrients. Culturing them on an agar or membrane surface accomplishes this; however, the total cell yield often is low, the cells probably are not physiologically uniform, and difficulty may occur in dislodging them. The concentration of cells ...

Economic cultivation of "thermophilic" Campylobacter spp.

Biphasic Microtiter Method for Campylobacter Recovery and Enumeration

We developed a biphasic method for recovery and enumeration of campylobacters. The biphasic system was composed of 96-well microtiter plates containing Campy–Line agar prepared 2¥ for all ingredients except for 1¥ agar (0.1 mL per well). Samples were prepared in either twofold or fivefold dilution series across separate 96-well plates and directly transferred (0.1 mL) to the agar-containing mic...

Biphasic culture of Arcobacter spp.

Arcobacter spp. have recently been genetically differentiated as a genus distinct from Campylobacter. Physiologically, Arcobacter spp. are aerotolerant bacteria, while Campylobacter spp. are microaerophilic. However, since Arcobacter spp. have been difficult to grow to high population densities in broth media, alternative culture techniques were investigated. A biphasic culture system was devel...